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Description
A simplified method for isolation of large numbers of defined nephron segments

Acknowledgements We thank the following Genentech colleagues for their critical feedback of this article: M

This is consistent with the dependence of phosphatase oxidation on pH, which increases with higher pH (63, 64)
These studies, combined with CKD patient renal biopsies, confirm renal ferroptosis as a key RF driver

Second, young FoxO4-null mice show no reproductive system impairment [29], indicating that FOXO4 does not play an important role in normal Leydig cells

The expression conditions and purification reported here have not been optimized, but yields of 16 mg/L of culture of purified disulfide bonded protein were obtained from the cytoplasm of E
