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Math, Karolinska Institutet (KI), Sweden Updates Copyright 2021 Nie, Liang, Shan, Wang, Mu, Zhou and Xia

Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template

318 , C304C327 (2020)

We are confident that NAPstars will drive the discovery and understanding of a broad range of novel NADP biology and its synthetic rewiring in the future

The increased expression of the Kras appears at 10 min