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Running a cycle for too short a time will yield minimal results, while running it for too long can lead to receptor desensitization or unnecessary cost

FineTest recommends to use ELISA curve fitting software and validate ELISA kit with 4 - pl curve fitting

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Interestingly, while the latter did not appear significant in hypothesis testing in 2 studies 40,56 , the accumulated data supports a positive association (OR: 3.532